Slice Model For Ex Vivo Study of Neural , Immune and Microbial Interactions of 4 Mouse Intestine 5

1 2 3 An Organotypic Slice Model For Ex Vivo Study of Neural, Immune and Microbial Interactions of 4 Mouse Intestine 5 Luke A. Schwerdtfeger, Elizabeth P. Ryan, Stuart A. Tobet 6 Department of Biomedical Sciences, School of Biomedical Engineering, and Department of 7 Environmental & Radiological Health Sciences 8 Colorado State University, Fort Collins, Colorado, 80523 9 10 11 Author Contributions: LAS conducted the experiments and wrote the manuscript. EPR and 12 SAT edited the manuscript and aided in experimental design. LAS and SAT formulated the 13 project. 14 15 Running heading: Ex vivo intestinal model 16 17 Correspondence: 18 Stuart A. Tobet 19 1617 Campus Delivery 20 Fort Collins, CO 80523-1617 21 Phone: 970 491 1672 22 Fax: 970 491 7907 23 email: [email protected] 24 Articles in PresS. Am J Physiol Gastrointest Liver Physiol (December 17, 2015). doi:10.1152/ajpgi.00299.2015